Investigation of temperature on catalase activity Essay Example for Free

Investigation of temperature on catalase activity Essay The aim of this experiment is to find out the effect of temperature on catalase or hydrogen peroxide. This will enable us to tell at what temperature hydrogen peroxide is most efficient. This (degradation) reaction will help us determine some of the catalases different attributes. HYPOTHESIS In this experiment it would be safe to hypothesise that no activity would take place at 1 to 20 degrees. It would be probable that a little activity would take place around 40 degrees. When the water gets to around 60+ the oxygen bubbles start to froth. It is almost certain that this will happen due to the behaviour of the atom. This means that the hotter the water gets the more active the water molecules become. Thus the colder the water gets the less active the water becomes. Another theory is that different things adapt according to their habitat. Therefore, catalase would be most active at around 37 degrees body temperature. This means that in this experiment catalase would be most active in the water bath that is at 40 degrees Celsius. METHOD Before the experiment could be started the following apparatus needed to be obtained: Cork Borer Water Baths at 3,20,40 and 60 degrees Celsius Potato Ruler Stopwatch Detergent Hydrogen Peroxide Now that all this apparatus has been obtained, it is possible to start the experiment. Eight test tubes will be required because the experiment is being done twice in each water bath. Use a marker pen to mark the specific temperature on the test tubes so as to make sure that there are no possible errors. Now place these test tubes in a test tube rack. Take the cork borer no. 4, and take a cylinder of potato using the cork borer. Do this until you have eight cylinders. If there are any cylinders that are uneven in size, then take the cylinder again using the cork borer. Now that you have good cylinders, cut them to equal length equal to 1.5cms. cubed. Once this has been done, put each potato cylinder in a test tube. Now it is almost time to start the experiment, so have a stop- watch at the ready. Pour 5cms cubed of hydrogen peroxide (H2O2) into each test tube. Now go to the first water bath that is 3 degrees Celsius and take a pipette and pour 10 drops of detergent into the first 2 test tubes (the test tubes that are labelled 3 degrees Celsius). Once you have done this, immediately start the stopwatch and simultaneously put the 2 tubes into the water bath. Repeat this process for the next 3 water baths. Although only 1 stopwatch has been used, the time will be fairly accurate since a delay has been created. When 5 minutes is up go to the first water bath with a ruler and measure the amount of froth on each test tube. RESULTS Experiment No.Temperature (oC)Height of Froth (cm)Average Height of Froth (cm) 133.33.15 2333.15 1203.43.5 2203.63.5 1406.56.0 2405.56.0 16032.95 2602.92.95 CONCLUSION In conclusion to this experiment, it is evident that catalase is most active at 40 degrees Celsius. There is a trend in a graph that shows the froth increases up towards 40 degrees Celsius and dips when reaching 60 degrees Celsius. It is not just a case of the Enzymes activity decreasing it becomes denatured shortly after it reaches its optimum level of activity. EVALUATION In this evaluation, the flaws and the limitations of this experiment will be outlined. The procedures in this experiment were very effective. Unlike many other experiments the control of the variables was without fault, because a machine was controlling them, i.e. the water bath. There were not many limitations of equipment, and the only way the equipment could have been improved would have been to have the experiment monitored by computer. All the sources of error in this experiment were with the non-variables. Firstly, there was no way of being sure that each potato was the exact same length or mass. A way of improving this would be using a guillotine-type device, meaning that the potatoes were at least the exact same length since  they were cut at equal angles all together. Another flaw is the amount of H2O2 poured into each separate test tube. This is cannot be improved upon unless done by machine. Other sources of error that could only have been improved upon by machine are height of froth, and amount of detergent. A major defect that could have affected the results is the timing. This is because of the delay system used. The only way that the delay system could have worked like clockwork is if it took the exact same time to measure froth as to pour detergent. There are two ways of improving this. The first is to make the experiment last longer. This would mean that it would not matter if there were a few seconds difference in the times, because no more froth could have been produced. The second is that if the experiment was timed separately for each bath.

Public Schools Vs. Post-Cold War Military Spending :: essays research papers

Public Schools vs. Post-Cold War Military Spending Even though the Cold War era is a distant memory, encased in glass forever like some museum piece, our government is still spending as if the Soviet Union was in its prime. If the arms race is a forgotten memory, then why is the military still spending 86% of what it was spending during the Cold War. It’s not that us Americans do not want a solid military, we just believe that our military is wasting billions of dollars at the expense of our children’s education and well being. 50 years ago our country, and the entire world was in disparate need of a strong U.S. military. We inflated our military budget, and gave the government and private businesses an enormous appetite. Without a major threat to the United States since the collapse of the Soviet Union, our government seems to be in competition with itself. 50 years ago we sent tons and tons of troops overseas to fight in a foreign land, while we pumped private businesses up with the manufacturing of military equipment. The need for such products and the need for an over-healthy military allowance is long gone. While we sunk billions after billions of taxpayer’s money on wars that we were boisterously proud to spend (it gave us all the prestige we could ever ask for), our Allies were investing in their children’s education and well being. The result now is that while we have the most elite military capabilities, our children’s educational level is extremely under developed. European an d Japanese children significantly outperform American children in math and upper-level reading (NATIONAL CATHOLIC REPORTER [NCR], 1999). This should not be too surprising, our children are studying history books that are from a decade ago. This creates difficult obstacles for our children to keep up on current politics and other global events. Also the over crowding of classrooms makes it extremely difficult for your child to get that personalized learning which may determine his or her ability to learn at all. Every child learns at a different rate and possibly in different ways, this makes it virtually impossible for teachers to tap into every child’s full learning potential. If your child needs that extra, personalized attention, but is not "defined" as handicapped their specific learning needs will most likely be overlooked and that may be the beginning of your child’s inability to learn.

History in the Making Essay

History†¦it’s who we are and where we come from. From cave men to cars that run on compost, history happens every day. Everything that has happened in history is absolutely fundamental in our current day existence. From the Revolutionary Era to the Cold War, America has come so far, and through the War on Terror, we continue to march on†¦ making every action history. The Revolutionary Era: After the British spent an extreme amount of money defending the Colonies against the French, they decided to shift some of the expenditures to the colonists. The result led to the Boston Tea Party in 1773 (Lib. Of Congress, p. 1), when the colonists rebelled against the British and their absurd tax on tea in order to re-coop defense expenditures. Reactions such as this led to the necessitation of order, which led to the Declaration of Independence, and the Revolutionary Era. The Constitution: As a result of deciding not to amend the current Articles of Confederation, a new frame of government was born. 55 delegates from age 26 to age 81 debated, redrafted and created what is now known as the Constitution of the United States. These articles clarified many topics such as how much control the government should have in certain areas, how representatives should be elected, and many other issues we still live by today. Since it’s publishing in 1787, there have been 27 amendments to the original version. Jeffersonianism: Derived from the term Jeffersonian, and named for its discoverer, the Jeffersonianism philosophy held that each man had a right to have a say-so in government, believed in the expansion of human freedom, and believed that every person had a right to be educated. It also supported reliance on the farmer and agriculture, instead of relying solely on History in the Making 3 industry to boost the economy. Hamiltonism, which followed Jeffersonianism, was believed to be an evil stigma to Jeffersonianism by Jeffersonians. Civil War: Brother against brother, the revolutionized nation could not decide whether or not the government should have control over the states, industrialization, and most importantly, slavery. The southern states, or the Confederate States of America, decided to take matters into their own hands and individualized themselves to make their own rules. This led to the Civil War, where one nation quarreled with its counterpart. Although the North won, losing over half a million soldiers did not make the impact any easier for anyone to handle. Reconstruction: After the period of the Civil War, a period of Reconstruction began. Attempts were made to solve differences between the Union and the 11 Confederate states that had seceded from the Union. Though opposition from the Radical Republicans was evident, the Reconstruction Act of 1867 was passed, and military districts in the South were re-established. The South had no choice but to accept the 14th and 15th amendments of the Constitution (39th Congress, p. 1). The New South: Although the days of slavery had ended, the Southern states continued to retain control over their civilization. With the abolition of slavery came anger from loss of control, which led to the birth of the Black Codes. The Black Codes allowed whites to control the newly freed slaves by regulating the type of work black people could do, the hours they worked, and prevented blacks from raising their own crops. Freedom was not freedom after all. Foreign Affairs: Lack of support from the European economies post Civil War distressed the cotton crops, as the producers became overstocked. The North added some restriction to the History in the Making 4 Southern ports, and our foreign allies soon found other sources to fulfill their needs, which hurt the South tremendously. Industrialization: As America advanced, so did the way work was performed. Not only did industrialization bring forth machinery, it also was the birth of transportation and communication. Andrew Carnegie’s steel industry revolutionized transportation. The rails made from refined steel created necessary use of the railroad system, as did communication with the use of the telegraphs and telephone systems (Feldmeth, p. 1). Progressive Era: Along with industrialization came those who felt that anything old was inefficient and useless. These reformers brought forth the Efficiency Movement, which argued that the economy, society and government were inefficient. Frederick Winslow Taylor coined the term â€Å"one best way† with the idea that there was one best way to fix everything. He, along with several other engineers improved industrial efficiency. World Wars: Taking place mostly in Europe, the First World War between the Allied Powers (France, the Russian Empire, the British Empire, Italy and the U. S) and the Central Powers (Austria-Hungary, the German Empire, Bulgaria and the Ottoman Empire) was a result of the assassination of the Austrian throne heir Archduke Franz Ferdinand and his wife. This event soon led to a full fledged war. The Treaty of Versailles would end the first war, and stake victory for the Allied Powers, but controversy over its contents would soon lead to World War II. The second war between the Allied Powers and the Axis Powers (Nazi Germany, Fascist Italy, and the Empire of Japan) would be the deadliest war to date, killing over 60 million people. This war resulted in victory for the Allied Powers. History in the Making 5 The Depression: In 1929 our nation suffered the worst market plunge to date. The stock market crash, in conjunction with the outrageous cost of WWII, led to great losses for many people. Soon, supply exceeded demand. Cities based heavy in industry-type operations, including those outside of the U. S. were hit especially hard. Farmers were also a target, as the price for crops fell between 40 and 50 percent. The New Deal, or restructuring of the economy, enforced by President Roosevelt, would help lead the nation to recovery. The Cold War: The Cold War, as dubbed by Bernard Baruch and Walter Lippmann, is a term used to describe the growing tensions between the US and the Soviet Union. It would be the longest war to date, lasting from the mid 1940’s to the early 1990’s. Post WWII, the two nations could not agree on how to reconstruct the post war world. Wars including the Korean War, the Cuban Missile Crises, and the Vietnam War were possible threats to lead into a third world war. The Cold War came to an end when Gorbachev launched his reform programs, Perestroika (reconstruction of the Soviet economy) and Glastnost (introduction of publicity and openness) (Wikipedia Staff, p. 1). It is almost impossible to summarize our nation’s history. So many events, far too many to place in a single composition of words, played key roles in the structure of how we live today. Needless to say, history continues to ensue every day. From our nation’s birth to the Cold War to the hanging of Saddam, America will continue to write a story we all should be so eager to read. References 1. Library of Congress (1998). Revolutionary Period. Retrieved in January 2007 from www. americaslibrary. gov/cgi. bin/page. cgiljb/revolt. 2. 39th Congress (1866). 14th Amendment to the US Constitution. Retrieved in January 2007 from www. nps. gov/archive/malu/documents/amend14. htm. 3. G. Feldmeth (2000). American Industrialization. Retrieved in January 2007 from www. home. earthlink. net/~gfeldmeth/lec. indust. html. 4. Wikipedia Staff (2001). Definitions for Perestroika and Glastnost. Retrieved in January 2007 from www. en. wikipedia. org.

Ethical And Ethical Implications Of Unethical Behavior

Here, in the Unites States, the American culture emphasize the latitude of freedom of expression in all forms, however in a 2006 Gallop poll of public perception of morals in the United States that 85% of respondents rated the current state of moral values as fair while 81% of respondents thought that the level of moral values is getting worse. So therefore, it is not surprising that in our media the level of unethical behaviors has become part or parcel of everyday lives. While the current population has resounding aired their frustrations via Gallop poll about the decline in public confidence in our various media forums, little has been done to improve the ethics in human communication. Many media forums have upheld the status quo that ratings matters over ethics. We have seen over and over that our contemporary ethical landscape is plagued with ethical widespread failure against the background of a culture rich with moral resources. We seem to have in everything we need in typical character and conduct, yet still flourishes on unethical practices and wrongdoings in almost every sector of work. Even though, we are faced with complexities of ethics in human communication, I believe the basics tenets, concepts and theories of ethics provide the framework necessary for working out one’s own moral or ethical code. Ethical principles are preexisting conditions necessary for the existence of any social community. Without ethical principles, it would be utter chaos and impossibleShow MoreRelatedEthical Implications Of Unethical Behavior1380 Words   |  6 PagesUnethical behavior is frequently a topic of controversy and discussion within our society. More often than not, this reaction is in response to a recent event which has brought to light the actions of others which we deem as morally wrong or unethical. 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